Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

6.9K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
6.9K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

869
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
869
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

22.4K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.4K
Types of RNA01:20

Types of RNA

5.6K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
5.6K
What is Gene Expression?01:36

What is Gene Expression?

8.4K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
8.4K
Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

15.1K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
15.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A paradoxical impact of alcohol on sleep-memory coupling.

Current biology : CB·2026
Same author

Tanycyte BMAL1 regulates high-fat diet weight gain and shapes arcuate neurogenesis in female mice.

Cell reports·2026
Same author

Monogenic epilepsies exhibit distinct sleep endophenotypes.

medRxiv : the preprint server for health sciences·2026
Same author

Clock gene signature predicts insomnia and links to sleep/circadian parameters.

Translational psychiatry·2026
Same author

Inducible, split base editors for in vivo cancer functional genomics.

Nature biotechnology·2026
Same author

Sleep-dependent clearance of brain lipids by peripheral blood cells.

Nature·2026

Related Experiment Video

Updated: Jun 5, 2025

Electrocorticographic Recording of Cerebral Cortex Areas Manipulated Using an Adeno-Associated Virus Targeting Cofilin in Mice
08:44

Electrocorticographic Recording of Cerebral Cortex Areas Manipulated Using an Adeno-Associated Virus Targeting Cofilin in Mice

Published on: February 21, 2021

4.3K

Modulation of RNA processing genes during sleep-dependent memory.

Yongjun Li1,2, Nitin S Chouhan1, Shirley L Zhang1

  • 1Howard Hughes Medical Institute and Chronobiology and Sleep Institute, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, United States.

Elife
|December 6, 2024
PubMed
Summary

Sleep-dependent memory consolidation in Drosophila involves specific RNA processing genes in anterior posterior neurons. Polr1F downregulation promotes sleep, while Regnase-1 is crucial for memory formation.

Keywords:
D. melanogasterDrosophilaRNA processingmemoryneurosciencesleep

More Related Videos

The Sleep Nullifying Apparatus: A Highly Efficient Method of Sleep Depriving Drosophila
06:06

The Sleep Nullifying Apparatus: A Highly Efficient Method of Sleep Depriving Drosophila

Published on: December 14, 2020

3.4K
Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice
08:58

Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice

Published on: June 19, 2019

9.7K

Related Experiment Videos

Last Updated: Jun 5, 2025

Electrocorticographic Recording of Cerebral Cortex Areas Manipulated Using an Adeno-Associated Virus Targeting Cofilin in Mice
08:44

Electrocorticographic Recording of Cerebral Cortex Areas Manipulated Using an Adeno-Associated Virus Targeting Cofilin in Mice

Published on: February 21, 2021

4.3K
The Sleep Nullifying Apparatus: A Highly Efficient Method of Sleep Depriving Drosophila
06:06

The Sleep Nullifying Apparatus: A Highly Efficient Method of Sleep Depriving Drosophila

Published on: December 14, 2020

3.4K
Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice
08:58

Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice

Published on: June 19, 2019

9.7K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Memory consolidation in Drosophila can be sleep-dependent or independent, influenced by food availability.
  • Anterior posterior (ap) alpha'/beta' (α'/β') neurons in the mushroom body (MB) are critical for sleep-dependent memory consolidation and increased sleep post-training.

Purpose of the Study:

  • To investigate the molecular mechanisms of sleep and memory consolidation initiation.
  • To analyze the transcriptome of ap α'/β' neurons after appetitive memory conditioning.

Main Methods:

  • Transcriptome analysis of ap α'/β' neurons 1 hour post-conditioning in fed vs. starved Drosophila.
  • Gene knockdown experiments in ap α'/β' neurons to assess sleep and memory phenotypes.
  • Analysis of Polr1F and Regnase-1 expression and function.

Main Results:

  • A subset of RNA processing genes showed differential expression in ap α'/β' neurons after conditioning.
  • Knockdown of Polr1F promoted sleep and increased translation, suggesting its downregulation aids sleep-dependent memory.
  • Regnase-1 knockdown impaired memory, but its role in sleep appeared developmental, not directly linked to sleep-dependent memory consolidation.

Conclusions:

  • RNA processing gene expression is modulated during sleep-dependent memory consolidation in Drosophila.
  • Polr1F downregulation in ap α'/β' neurons contributes to increased sleep associated with memory formation.
  • Regnase-1 plays a significant role in memory consolidation, though its downregulation during sleep-dependent memory requires further investigation.