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A conserved nuclear factor YC subunit, NF-YC3, is essential for arbuscule development.

Kun Xie¹, Yuhan Ren¹, Yujuan Huang¹

¹State Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China.

The Plant Journal : for Cell and Molecular Biology

|December 6, 2024

Summary

OsNF-YC3 is crucial for arbuscular mycorrhizal (AM) symbiosis in plants, enhancing phosphate uptake. Its regulation and downstream targets are key to improving crop nutrient efficiency.

Keywords:

Oryza sativa OsNF‐YC3 PHR transcription factors arbuscular mycorrhizal symbiosis nuclear factor

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Area of Science:

Plant biology
Mycology
Genetics

Background:

Arbuscular mycorrhizal (AM) fungi symbiosis is vital for plant adaptation to environmental stresses, particularly phosphate deficiency.
Understanding key genes in AM symbiosis is crucial for developing crops with improved nutrient uptake.
OsNF-YC3, a nuclear factor YC subunit gene, is specifically expressed in arbuscule-containing cells.

Purpose of the Study:

To investigate the role of OsNF-YC3 in arbuscular mycorrhizal (AM) symbiosis.
To identify the regulatory mechanisms and downstream targets of OsNF-YC3.
To explore the potential of OsNF-YC3 in enhancing plant nutrient uptake efficiency.

Main Methods:

Gene expression analysis
Gene knockout and overexpression studies
Chromatin immunoprecipitation sequencing (ChIP-seq)
Yeast three-hybrid assays

Main Results:

OsNF-YC3 knockout mutants exhibited stunted arbuscule development and reduced phosphorus accumulation.
Overexpression of OsNF-YC3 enhanced mycorrhization and phosphate (Pi) uptake efficiency.
OsNF-YC3 is directly regulated by OsPHRs and targets genes like OsPT11 involved in arbuscule development.
OsNF-YC3 interacts with OsNF-YA11 and OsNF-YB11, with OsNF-YA11 also being essential for arbuscule development.

Conclusions:

OsNF-YC3 plays an essential role in regulating AM symbiosis and arbuscule development.
OsNF-YC3 and its interacting subunit OsNF-YA11 are key components of the regulatory network controlling AM symbiosis.
These findings provide insights for breeding crops with enhanced nutrient uptake capabilities.