Related Experiment Video

Updated: Jun 5, 2025

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses
11:48

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses

Published on: April 4, 2019

15.4K

Structural and molecular properties of mumps virus inclusion bodies

Hiroshi Katoh1, Ryuichi Kimura2,3, Tsuyoshi Sekizuka4

  • 1Department of Microbiology, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.

Science Advances
|December 6, 2024
PubMed

Insights

Mumps virus inclusion bodies (IBs) form liquid organelles through phase separation. Host G-quadruplex RNAs (G4-RNAs) interact with viral proteins, playing a key role in forming these essential viral structures.

Area of Science:

  • Virology
  • Molecular Biology
  • Cell Biology

Background:

  • Mononegaviruses synthesize viral RNA within cytoplasmic inclusion bodies (IBs).
  • Mumps virus (MuV), a Paramyxoviridae member, causes mumps and relies on IBs for replication.

Purpose of the Study:

  • To investigate the structural properties and formation mechanism of MuV inclusion bodies (IBs).
  • To identify host factors involved in MuV IB assembly.

Main Methods:

  • Super-resolution microscopy to analyze MuV IB structure.
  • Spatial transcriptome analysis to identify host RNAs in IBs.
  • In vitro phase separation assays to study protein-RNA interactions.

Main Results:

  • MuV IBs exhibit liquid organelle properties, driven by liquid-liquid phase separation.
  • Nucleocapsid and phospho (P)-proteins form a cage-like structure, with viral polymerase and RNA localized within.
  • Host G-quadruplex RNAs (G4-RNAs) are concentrated in MuV IBs and enhance P-protein condensation.

Conclusions:

  • MuV IBs possess a distinct cage-like structure indicative of liquid organelle behavior.
  • Host G4-RNAs are crucial components that facilitate the formation and condensation of MuV IBs.

More Related Videos

In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation07:24

In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation

Published on: March 27, 2016

9.0K
Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo08:29

Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo

Published on: October 21, 2014

12.2K

Related Concept Videos

Viral Structure00:56

Viral Structure

Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
61.7K
Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
3.4K
Cell Inclusions01:27

Cell Inclusions

Prokaryotic cells possess a variety of inclusions that play crucial roles in nutrient storage, metabolic processes, and environmental adaptation. These structures enable bacteria to thrive under fluctuating environmental conditions by storing essential resources and optimizing their metabolic efficiency.Carbon Storage: Poly-β-Hydroxybutyric Acid and Glycogen GranulesBacteria frequently store excess carbon in specialized granules. Poly-β-hydroxybutyric acid (PHB) granules are lipid...
2
Fluid Mosaic Model01:19

Fluid Mosaic Model

Scientists identified the plasma membrane in the 1890s and its principal chemical components (lipids and proteins) by 1915. The model for plasma membrane structure, proposed in 1935 by Hugh Davson and James Danielli, was the first model to be widely accepted in the scientific community. The model was based on the plasma membrane's "railroad track" appearance in early electron micrographs. Davson and Danielli theorized that the plasma membrane's structure resembled a sandwich...
11.4K
Intracellular Movement of Viruses and Bacteria01:10

Intracellular Movement of Viruses and Bacteria

Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
2.8K