Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

AI-driven discovery of GPNMB CAR T cells as a multi-cancer therapy.

Cell·2026

Same author

Ten-Year Outcomes after CAR T-Cell Therapy for B-Cell Lymphomas.

The New England journal of medicine·2026

Same author

Bioinspired oxidized mRNA lipid nanoparticles for ex vivo engineering of chimeric antigen receptor macrophages targeting solid tumors.

Bioengineering & translational medicine·2026

Same author

A generalizable system for antigenic peptide targeting across HLA-I allotypes.

bioRxiv : the preprint server for biology·2026

Same author

Kidney Transplantation in Two Highly Sensitized Candidates after CAR T-Cell Therapy.

The New England journal of medicine·2026

Same author

Rab5 improves CAR T cell efficacy via reducing fratricide and maintaining surface CAR levels.

The Journal of experimental medicine·2026

Same journal

Genetic Biosensor for Optimizing Double-Stranded RNA Production by Bacteria.

ACS synthetic biology·2026

Same journal

Heterologous Expression of an Abandoned Termite Mound Fungus Gene Cluster Reveals a Protective Aldehyde-Alcohol Cycle and a Candidate Termiticidal Metabolite.

ACS synthetic biology·2026

Same journal

A Framework for the In Vivo Production of Extensively Engineered Thiopeptides.

ACS synthetic biology·2026

Same journal

A Highly Stringent Split Intein-Mediated DHFR Selectable Marker Enables Efficient Development of High-Producing CHO Cells for Therapeutic Proteins.

ACS synthetic biology·2026

Same journal

Breaking the Stability-Activity-Selectivity Trilemma in Unspecific Peroxygenase through Computation-Based Cross-Regional Combinatorial Mutagenesis.

ACS synthetic biology·2026

Same journal

Sequential Plasmid Curing and Genome Editing in <i>Escherichia coli</i> Nissle 1917.

ACS synthetic biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 5, 2025

piggyBac Transposon System Modification of Primary Human T Cells

piggyBac Transposon System Modification of Primary Human T Cells

Published on: November 5, 2012

Stem Loop Mediated Transgene Modulation in Human T Cells.

David Mai^1,2, Carly Harro², Aabir Sanyal¹

¹Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.

ACS Synthetic Biology

|December 6, 2024

Summary

This summary is machine-generated.

This study introduces a novel method for controlling gene expression using RNA stem loops in human T cells, eliminating the need for external drug inputs. This cell-autonomous system enables precise regulation of transgenes for advanced applications.

More Related Videos

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Published on: August 13, 2013

Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function

Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function

Published on: November 4, 2016

Related Experiment Videos

Last Updated: Jun 5, 2025

piggyBac Transposon System Modification of Primary Human T Cells

piggyBac Transposon System Modification of Primary Human T Cells

Published on: November 5, 2012

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Published on: August 13, 2013

Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function

Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function

Published on: November 4, 2016

Area of Science:

Molecular Biology
Immunology
Synthetic Biology

Background:

Gene expression control is crucial for various applications but often relies on external stimuli like drugs.
Existing methods for transgene regulation can be cumbersome and lack cell-autonomous control.

Purpose of the Study:

To develop a cell-autonomous system for transgene regulation in primary human T cells.
To utilize RNA stem loop structures and endogenous RNA-binding proteins for programmable gene control.

Main Methods:

Engineered RNA stem loop structures were incorporated into the 3' untranslated regions (UTRs) of transgenes.
The system leveraged the activity of Regnase-1 and Roquin-1 RNA-binding proteins for gene regulation.
The system was tested and demonstrated in primary human T cells, including CAR-T cells.

Main Results:

Achieved transgene repression via Regnase-1 and Roquin-1 activity.
Demonstrated dynamic transgene upregulation upon stimulation.
Showcased orthogonal tunability of the regulatory system.
Successfully modulated payloads in CAR-T cells.

Conclusions:

RNA stem loop structures offer a powerful tool for cell-autonomous transgene regulation.
Leveraging endogenous regulatory machinery in T cells provides a promising approach for gene control.
RNA structure presents a valuable regulatory layer for synthetic biology applications.