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Updated: Jun 5, 2025

Luminophore Formation in Various Conformations of Bovine Serum Albumin by Binding of GoldIII
Published on: August 31, 2018
Interaction between newly synthesized surface-active ionic liquids with pharmaceutically active anion and bovine
Artashes A Khachatrian1, Timur A Mukhametzyanov1, Ramazan Z Salikhov1
1Department of Physical Chemistry, Kazan Federal University, Kremlyovskaya str.18, Kazan 420008, Russian Federation.
Abstract:
This work describes the synthesis and properties of new surface active (octyl-, and dodecyl-) imidazolium and choline-based ionic liquids with pharmaceutically active 5-fluorouracil anion (SAAPI-IL). The effect of the novel SAAPI-IL on the secondary and tertiary structure of bovine serum albumin (BSA) was studied using circular dichroism (CD) spectroscopy. The binding constants of BSA with SAAPI-IL were estimated from the fluorescence quenching of BSA. The influence of the length of the alkyl chain SAAPI-IL on the binding constant with BSA was analyzed. The localization of ions on BSA was estimated using the molecular docking method. Also, the effect of alkyl substituent length on the aggregation tendency of SAAPI-IL was studied by dynamic light scattering (DLS). Cytotoxicity and selectivity of SAAPI-ILs on cancer and normal cell lines were compared to molecular 5-fluorouracil and API-IL without the alkyl substituents. Surprisingly, increasing the length of the alkyl substituent did not increase the binding of SAAPI-IL to BSA. On the other hand, [C8Ch][FU] showed selectivity against A 549 cell line, which 5-fluorouracil does not possess.
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