Protocol for single sensillum recording from labial olfactory sensory fields in spotted lanternfly

Area of Science:

• Entomology
• Neuroscience
• Chemical Ecology

Background:

• The spotted lanternfly (SLF), Lycorma delicatula, is an invasive sap-feeding planthopper with significant agricultural impact.
• Understanding SLF host plant selection, aggregation, and mating behaviors is critical for effective pest management.
• Olfactory receptor neurons (ORNs) play a key role in detecting odorants for these behaviors.

Purpose of the Study:

• To present a detailed protocol for measuring electrophysiological responses of SLF labial ORNs to various odorants.
• To enable researchers to assess the sensitivity, specificity, and response dynamics of SLF ORNs.

Main Methods:

• Single sensillum recording technique applied to SLF labial ORNs.
• Protocol covers odorant cartridge preparation, labium mounting, electrophysiology rig setup, and data acquisition.
• Standardized procedures for recording neuronal responses to a range of odorants.

Main Results:

• The protocol allows for quantitative measurement of ORN responses to odorants.
• Data obtained can reveal specific odorant sensitivities and response patterns of SLF ORNs.
• This method provides insights into the olfactory mechanisms underlying SLF behavior.

Conclusions:

• The presented protocol is essential for studying SLF olfaction.
• This research facilitates a deeper understanding of SLF's interaction with its environment.
• The findings contribute to developing targeted strategies for SLF pest control.