Protocol to measure glucose utilization in mouse tissues using radiolabeled 2-deoxy-D-glucose

Cyun-Ming Wu ¹, Hao-Yun Li ¹, Wen-Yi Li ²

¹Department of Medicine, College of Medicine, National Taiwan University, Taipei 100, Taiwan.
²Division of Nephrology, Department of Internal Medicine, National Taiwan University Hospital, Yunlin Branch, Yunlin 640, Taiwan.
³Cardiovascular Center, National Taiwan University Hospital, Yunlin Branch, Yunlin 640, Taiwan.
⁴Division of Nephrology, Department of Internal Medicine, National Taiwan University Hospital, HsinChu Branch, HsinChu 300, Taiwan.
⁵Division of Nephrology, Department of Pediatrics, National Taiwan University Hospital, Taipei 100, Taiwan.
⁶PhD Program in Translational Medicine, National Taiwan University and Academia Sinica, Taipei 100, Taiwan.
⁷Department of Medicine, College of Medicine, National Taiwan University, Taipei 100, Taiwan; Graduate Institute of Medical Genomics and Proteomics, National Taiwan University, Taipei 100, Taiwan.
⁸Division of Endocrinology and Metabolism, Department of Internal Medicine, National Taiwan University Hospital, Taipei 100, Taiwan.
⁹Graduate Institute of Medical Genomics and Proteomics, National Taiwan University, Taipei 100, Taiwan.
¹⁰Biological Sciences Division, University of Chicago, Chicago, IL 60637, USA.
¹¹Institute of Molecular Medicine, National Taiwan University, Taipei 100, Taiwan.
¹²Division of Cardiology, Department of Internal Medicine, E-Da Hospital, I-Shou University, Kaohsiung 824, Taiwan; Division of Cardiology, Department of Internal Medicine, E-Da Dachang Hospital, I-Shou University, Kaohsiung 807, Taiwan; School of Medicine, College of Medicine, I-Shou University, Kaohsiung 824, Taiwan.
¹³PhD Program in Translational Medicine, National Taiwan University and Academia Sinica, Taipei 100, Taiwan; Division of Endocrinology and Metabolism, Department of Internal Medicine, National Taiwan University Hospital, Taipei 100, Taiwan; Graduate Institute of Medical Genomics and Proteomics, National Taiwan University, Taipei 100, Taiwan; Institute of Biomedical Sciences, Academia Sinica, Taiwan 100, Taiwan.

⁰Department of Medicine, College of Medicine, National Taiwan University, Taipei 100, Taiwan.

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December 7, 2024

View abstract on PubMed

Summary

This study details a protocol for measuring glucose utilization in mice using radiolabeled 2-deoxy-D-glucose (2DG). The method quanties glucose metabolism in various tissues, offering insights into cellular energy processes.

Area Of Science

Biochemistry
Physiology
Metabolic Research

Background

2-deoxy-D-glucose (2DG) is a glucose analog.
Hexokinase converts 2DG to 2DG-6P in glycolysis.
2DG measures glucose uptake, while 2DG-6P indicates glucose utilization.

Purpose Of The Study

To present a protocol for measuring glucose utilization in mouse tissues.
To quantify metabolic activity using radiolabeled 2DG.
To establish a method for assessing tissue-specific glucose metabolism.

Main Methods

Preparation of mice and chemical reagents.
Blood extraction and chemical administration.
Tissue dissolution and radioactivity measurement.
Calculation of glucose utilization via the trapezoid rule.

Main Results

A detailed protocol for measuring glucose utilization in vivo.
Demonstration of quantifying 2DG-6P formation in various tissues.
Method allows for assessment of metabolic flux.

Conclusions

The presented protocol enables accurate measurement of glucose utilization in mouse tissues.
This method provides a valuable tool for studying metabolic pathways.
The findings contribute to understanding glucose metabolism and its alterations in disease.

Keywords:

Health Sciences Metabolism Metabolomics