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Target-driven functionalized DNA hydrogel capillary sensor for SARS-CoV-2 dual-mode detection.

Jing-Bo Jiao1, Qing Kang2, Shu-Xin Cui2

  • 1State Key Laboratory of Food Nutrition and Safety, Engineering Research Center of Food Biotechnology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China; College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.

Talanta
|December 7, 2024
PubMed
Summary

A novel DNA hydrogel capillary sensor offers sensitive dual-mode detection of SARS-CoV-2 RNA. This method utilizes cascade signal amplification and nanoparticles for rapid and reliable diagnostics in clinical samples.

Keywords:
Capillary sensorCascaded signal amplificationDual-mode detectionFunctionalized DNA hydrogelSARS-CoV-2

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Analytical Chemistry

Background:

  • The COVID-19 pandemic, caused by SARS-CoV-2, continues to pose significant global health and economic challenges.
  • Accurate and rapid detection of SARS-CoV-2 is crucial for disease control and prevention.

Purpose of the Study:

  • To develop a sensitive and versatile dual-mode sensor for the detection of SARS-CoV-2 RNA.
  • To leverage functionalized DNA hydrogels and nanoparticle-based signal amplification for enhanced diagnostic capabilities.

Main Methods:

  • Development of a target-driven DNA hydrogel capillary sensor incorporating carbon-coated cobalt-manganese, prussian blue, and platinum nanoparticles (MnCo@C-Pt-PB NPs).
  • Utilizing cascade signal amplification triggered by target RNA to release MnCo@C-Pt-PB NPs.
  • Employing a dual-mode detection strategy involving colorimetric changes (TMB oxidation) and photothermal signals under near-infrared irradiation.

Main Results:

  • The sensor demonstrated a wide detection range for target RNA from 100 aM to 100 pM, with a low detection limit of 100 aM.
  • The method showed reliable performance in detecting SARS-CoV-2 RNA in oropharyngeal swab samples.
  • Dual-mode detection achieved through catalytic activity and photothermal properties of the nanoparticles.

Conclusions:

  • The developed functionalized DNA hydrogel capillary sensor offers a sensitive, convenient, and versatile platform for SARS-CoV-2 detection.
  • This technology holds promise for applications in environmental safety, molecular diagnostics, and disease prevention.