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Updated: Jun 21, 2026

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus
Published on: November 30, 2014
Downregulation of the DNA replication pathway genes stimulate reproductive diapause preparation in the cabbage
Shuang Guo1, Hao-Min An2, Zhong Tian3
1Hubei Key Laboratory of Insect Resources Utilization and Sustainable Pest Management, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Abstract:
Diapause is a prevalent strategy employed by insects to survive adverse environmental conditions, by halting development and reducing metabolic activity. Although the suppression of DNA replication aligns logically with these reduced developmental and physiological activities, the role of DNA replication in regulating insect diapause remains largely unknown. In this study, we used the cabbage beetle, Colaphellus bowringi, to investigate the role of DNA replication pathway in regulating reproductive diapause. Transcriptome analysis identified DNA replication as a key pathway during diapause preparation in female adults. Fourteen DNA replication genes were isolated, encompassing essential stages of DNA replication, including DNA unwinding, primer synthesis, and DNA synthesis. These genes exhibited consistently reduced expression in diapause females compared to those in reproductive females. RNA interference knockdown of these genes in reproductive female adults resulted in 11 out of 14 gene depletions, manifesting typical diapause traits such as suppressed vitellogenesis, arrested ovary growth, and increased lipid accumulation. Furthermore, we demonstrated 20-hydroxyecdysone (20E), through canonical signaling pathway, regulates the differential expression of DNA replication genes between reproductive and diapause states. Our findings suggest 20E deficiency suppresses DNA replication to induce reproductive diapause, and highlighting the DNA replication process as a potential target for pest management.
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