Related Experiment Video
Updated: Jun 5, 2025
08:05
Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture
Published on: September 29, 2017
19.1K
Double-alternating injectable multifunctional hydrogel based on chitosan for skin wound repair
Yongyi Yang1, Rong Liu2, Li Xie2
1Department of Pharmacy, Chengdu University, Chengdu, Sichuan 610106, China.
International Journal of Biological Macromolecules
|December 7, 2024
Summary
A novel chitosan-based injectable hydrogel enhances wound healing by combining dipotassium glycyrrhizinate and bioactive glass. This double-alternating hydrogel promotes cell growth and tissue regeneration for improved skin healing.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Wound Healing
Background:
- Injectable hydrogels are crucial for irregular wound healing.
- Chitosan-based hydrogels offer promising therapeutic potential.
- Developing advanced hydrogel systems is essential for improving clinical outcomes.
Purpose of the Study:
- To develop and characterize a novel chitosan-based double-alternating injectable hydrogel for wound healing.
- To evaluate the physicochemical, mechanical, and biological properties of the hydrogel.
- To assess the in vivo efficacy of the hydrogel in promoting full-thickness skin wound healing.
Main Methods:
- Fabrication of a double-alternating hydrogel skeleton (COG) using carboxymethyl chitosan (CMCS) and oxidized gellant (OGG) via Schiff base bonds.
- Loading dipotassium glycyrrhizinate (DPG) and bioactive glass (BG) into the hydrogel to form COG2-D10 and COG2-B0.5.
- Systematic investigation of rheological behaviors, injectability, cytocompatibility, antibacterial, and anti-inflammatory activities.
- In vivo evaluation of full-thickness skin wound healing efficacy.
Main Results:
- The developed hydrogel exhibited a stable network structure, good toughness, injectability, and excellent adhesive properties.
- The hydrogel demonstrated efficient antibacterial, anti-inflammatory, hemostatic, and biocompatible properties.
- The double-alternating hydrogel significantly enhanced drug efficacy, promoted cell migration and proliferation, accelerated collagen deposition and neovascularization, leading to improved wound healing (p < 0.01).
Conclusions:
- The chitosan-based double-alternating injectable hydrogel possesses desirable properties for wound healing applications.
- This hydrogel formulation effectively promotes skin wound healing by enhancing therapeutic effects and tissue regeneration.
- The double-alternating hydrogel therapy strategy presents a promising new approach for clinical skin wound treatment.
