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Updated: Jun 5, 2025

Author Spotlight: Advancements in iPSCs and Genetic Disease Research
Published on: October 20, 2023
Generation of human induced pluripotent stem cell lines (iPSC) from adipose-derived mesenchymal stromal cells from
Mélanie Velier1, Romain Appay2, Robin Arcani3
1Aix-Marseille Univ, C2VN, INSERM 1263, INRAE1260, Marseille, France; Culture and Cell Therapy Laboratory, INSERM CIC BT 1409, Hôpital de la Conception, Assistance Publique Hôpitaux de Marseille (AP-HM), Marseille, France.
Abstract:
Systemic sclerosis (SSc) is a rare and complex connective tissue disease associated with high morbidity and mortality. SSc is characterized by ischemic vasculopathy, cutaneous and visceral fibrosis and a dysimmune state (Denton and Khanna, 2017; Volkmann et al., 2023; Barnes and Mayes, 2012). We have derived induced pluripotent stem cell (iPSC) lines from two SSc patients aged 38 and 67 years with severe vascular phenotype. These iPSC lines expressed pluripotent markers, exhibited normal and stable genome, and differentiated into trilineage embryonic layers in teratoma formation assays. These SSc-specific iPSC lines can be differentiated into endothelial cells, providing a valuable model to elucidate vascular dysfunction and develop personalized therapeutic approaches.
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