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Updated: May 12, 2026
Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water
Published on: June 9, 2014
A press-actuated slidable microfluidic colorimetric biosensor for Salmonella detection utilizing nickel mesh sheet
Jing Yuan1, Lei Wang1, Hong Duan2
1Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100083, China.
Abstract:
A press-actuated slidable microfluidic colorimetric biosensor was designed for rapid, sensitive and multi-channel detection of Salmonella. The nickel mesh sheet (NMS) modified with capture antibodies was employed for capturing target bacteria, and metal organic frameworks decorated with palladium (Pd) and platinum (Pt) nanoparticles (MIL-88@Pd/Pt NPs) modified with detection antibodies were used for amplifying colorimetric signals. The capture efficiency of the immune NMS reached 83 %, and the detection limit of this colorimetric biosensor was 35 CFU/mL in 20 min. The average recoveries for Salmonella in spiked chicken meats ranged from 92.2 % to 102.5 % with a variation from 3.7 % to 7.2 %. The press-actuated slidable microfluidic chip was elaboratively developed with multiple functions, including mixing, separation, washing, catalysis and detection.
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