Enhancing non-viral DNA delivery systems: Recent advances in improving efficiency and target specificity

Area of Science:

• Biotechnology
• Molecular Biology
• Gene Therapy

Background:

• DNA-based therapies face limitations including poor stability, integration, transfection, and targeted delivery.
• Non-viral delivery systems offer a promising alternative to overcome these challenges.

Purpose of the Study:

• To review recent advancements in non-viral DNA delivery systems.
• To highlight strategies for enhancing targeted DNA delivery and therapeutic efficiency.

Main Methods:

• Exploration of diverse administration routes for optimal cellular and organ uptake.
• Modification of delivery vectors with cell-specific ligands for targeted cellular recognition and improved biodistribution.

Main Results:

• Optimized administration routes improve cellular uptake and gene delivery efficiency.
• Ligand modification enhances specificity, biodistribution, and targeted DNA delivery.
• Progress in these strategies is accelerating clinical translation of DNA therapies.

Conclusions:

• Non-viral delivery systems are crucial for advancing DNA-based therapies.
• Targeted delivery strategies are key to improving therapeutic outcomes and clinical applications.