Related Experiment Video
Updated: Jun 5, 2025

Detection and Removal of Tooth-Colored Composite Resin Using the Fluorescence-Aided Identification Technique
Published on: July 27, 2022
Effect of coffee roasting level on tooth discoloration
Soyeon Kim1, Sri Larnani1, Noha Taymour2
1Department of Oral Anatomy and Dental Research Institute, School of Dentistry, Seoul National University.
Purpose:
Coffee consumption is a well-known contributor to tooth discoloration, and the extent of staining is influenced by the chemical composition of the coffee. This study investigated the associations of coffee roasting level, chlorogenic acid (CGA) content, absorbance level, and their combined effects with tooth discoloration.
Methods:
Bovine tooth enamel specimens were immersed in light, medium, and dark roasts of four coffee types (two Arabica and two Robusta coffees) for 72 h. High-performance liquid chromatography (HPLC) was used to measure CGA content, absorbance levels were estimated by using pigment concentration, and discoloration was assessed by spectrophotometry. The data were analyzed with the Friedman test.
Results:
Medium roasts induced the greatest discoloration, and tooth specimens immersed in Ethiopia Arabica exhibited the greatest color difference based on CIEDE2000 (ΔE00 at 72 h: 13.51 ± 4.63). Light roasts induced the least staining, despite having the highest CGA content. Dark roasts showed the highest absorbance, indicating a higher pigment concentration. Friedman analysis revealed a significant difference in color change in relation to roasting level for all coffee types.
Conclusion:
The present findings indicate that tooth discoloration is caused by the complex interaction of CGA, melanoidins, and roasting level. Because of the interplay of these factors, medium roasting had the greatest effect on discoloration.
More Related Videos
Related Concept Videos
Teeth
In the bud stage, the tooth germ (an aggregation of cells) starts to form in the developing jawbone. During the cap stage, the tooth germ differentiates into enamel organ, dental papilla, and dental sac, which will later develop into the tooth's enamel, dentin...
Changes in Skin Color: Clinical Perspectives
Albinism
Albinism is a genetic disorder that affects (completely or partially) the coloring of skin, hair, and eyes. The defect is primarily...
Tooth Anatomy
The Crown, Neck, and Root
The visible part of the tooth is referred to as the crown. It's covered by enamel, the hardest substance in the human body. The crown is uniquely shaped for each type of tooth, allowing for different functions such as cutting, tearing, or...
Experimental Designs
Pigmentation
Melanin occurs in two primary forms: eumelanin that provides black and brown pigment and pheomelanin that provides red color. Dark-skinned individuals produce more melanin than those with pale...
Oxidations of Aldehydes and Ketones to Carboxylic Acids
Aldehydes readily undergo oxidation in strong oxidizing agents such as potassium permanganate and chromic acid. The oxidation can also be carried out using mild oxidizing agents such as silver oxide. In fact, aldehydes can be easily oxidized...

