Unlocking the potential of chitosan in immunoassay sensor

Using new materials to improve detection accuracy and efficiency is important to broaden the application of immunoassay sensors. Among numerous materials for improving sensors, chitosan, as a natural polysaccharide, has excellent biocompatibility, mechanical adjustability, stimulation sensitivity and porous structure, which holds significant potential for enhancing the performance of immunoassay sensors. However, at present, there are few systematic reviews on the application of chitosan in immune sensors. In this paper, the principle of immunoassay is discussed systematically, we reviewed the recent development of chitosan enhancement strategies in various immunoassay sensors, including surface plasmon resonance immunoassay sensors, colorimetric immunoassay sensors, electrochemical immunoassay sensor, electrochemical luminescence immunoassay sensors are reviewed. Focused on the theoretical basis of improving sensor performance in immunoassay by use chitosan, as well as the various functions and applications of chitosan, and discussed how to solve the challenges of immunoassay sensors by using chitosan and the future research trend. By providing a robust foundation for the development of more efficient detection platforms, it provides insights for advancing the use of chitosan in the detection of complex biological samples. This is crucial for promoting the widespread application of immunoassay sensors with high performance in clinical diagnosis, environmental monitoring and food safety.