Mueller matrix polarimetry for quantitative evaluation of the Achilles tendon injury recovery

Achilles tendon injuries, as a widely existing disease, have attracted a lot of research interest. Mueller matrix polarimetry, as a novel label-free quantitative imaging method, has been widely used in various applications of lesion identification and pathological diagnosis. However, focusing on the recovery process of Achilles tendon injuries, current optical imaging methods have not yet achieved the label-free precise identification and quantitative evaluation. In this study, using Mueller matrix polarimetry, various Achilles tendon injury samples were characterized specifically, and the efficacy of different recovery schemes was evaluated accordingly. Experiments indicate that injured Achilles tendons show less phase retardance, larger diattenuation, and relatively disordered orientation. The combination of experiments with Monte Carlo simulation results illustrate the microscopic mechanism of the Achilles tendon recovery process from three aspects, that is, the increased fiber diameter, a more consistent fiber orientation, and greater birefringence induced by more collagen protein. Finally, based on the statistical distribution of polarization measurements, a polarization specific characterization parameter was extracted to construct a label-free image, which cannot only intuitively show the injury and recovery of Achilles tendon samples, but also give a quantitative evaluation of the treatment.