Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The membrane attack complex of complement: C5b-8 complex as accelerator of C9 polymerization.

J Tschopp, E R Podack, H J Müller-Eberhard

    Journal of Immunology (Baltimore, Md. : 1950)
    |January 1, 1985
    PubMed
    Summary

    The complement protein C5b-8 complex significantly accelerates C9 polymerization, forming tubular structures within minutes. The ratio of C9 to C5b-8 influences the formation and structure of the final C5b-9 complex.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The molecular mechanism of lymphocyte-mediated tumor cell lysis.

    Immunology today·2014
    Same author

    Perforin-2 restricts growth of Chlamydia trachomatis in macrophages.

    Infection and immunity·2013
    Same author

    CD30L/CD30 is critical for maintenance of IL-17A-producing γδ T cells bearing Vγ6 in mucosa-associated tissues in mice.

    Mucosal immunology·2013
    Same author

    Cell-secreted Gp96-Ig-peptide complexes induce lamina propria and intraepithelial CD8+ cytotoxic T lymphocytes in the intestinal mucosa.

    Mucosal immunology·2009
    Same author

    RELATION OF A beta(1)-GLYCOPROTEIN OF HUMAN SERUM TO THE COMPLEMENT SYSTEM.

    The Journal of experimental medicine·2009
    Same author

    The c3-activator system: an alternate pathway of complement activation.

    The Journal of experimental medicine·2009

    Area of Science:

    • Immunology
    • Complement System Biology

    Background:

    • Complement protein C9 polymerization is a crucial step in the formation of the membrane attack complex (MAC).
    • Spontaneous C9 polymerization is slow, requiring days to complete.
    • The tetramolecular complex C5b-8 is known to interact with C9.

    Purpose of the Study:

    • To investigate the role of the C5b-8 complex in accelerating C9 polymerization.
    • To determine how the molar ratio of C9 to C5b-8 affects the formation of tubular poly C9.
    • To examine C9 polymerization in the context of whole serum and its effect on C5b-9 complex ultrastructure.

    Main Methods:

    • Investigated spontaneous C9 polymerization kinetics at 37°C.
    • Studied C9 polymerization induced by the C5b-8 complex using phospholipid vesicles.

    Related Experiment Videos

  • Analyzed the formation of tubular and non-tubular poly C9 using SDS-resistance assays.
  • Generated C5b-9 complexes on rabbit erythrocytes using human serum and assessed C9 polymerization.

    • Main Results:

    • C5b-8 dramatically accelerated C9 polymerization, completing it within 10 minutes compared to over 3 days spontaneously.
    • The molar ratio of C9:C5b-8 was critical; ratios of 9:1 or 12:1 resulted in 72% SDS-resistant tubular poly C9.
    • Lower ratios favored non-tubular poly C9 formation.
    • In erythrocytes, high serum concentration or C9-supplemented serum led to up to 40% SDS-resistant tubular poly C9, with incomplete polymerization observed otherwise.

    Conclusions:

    • The C5b-8 complex acts as a potent accelerator for C9 polymerization.
    • The relative concentrations of C5b-8 and C9 dictate the ultrastructure of the C5b-9 complex, specifically the formation of tubular poly C9.
    • Understanding these dynamics is key to comprehending complement-mediated cell lysis.