Polarised light scanner for digital pathology

Digital pathology is rapidly transforming diagnostic pathology by allowing remote work and integration of artificial intelligence solutions. Nevertheless, certain technical issues remain to be resolved. Notably, digital images captured by conventional scanners cannot be subjected to polarised light analysis [1]. We have therefore studied if images obtained using the Glissando POL Brightfield and Polarised Light Scanner are comparable to those obtained using conventional polarised light microscopy. Hematoxylin and eosin stained sections from 75 cases, including cases of amyloidosis, periprosthetic membranes, foreign body granulomas, gout, pseudogout, and breast tissues with calcium oxalate crystals were examined using both, a polarised light microscope and the Glissando POL scanner. Representative digital images were acquired for comparison. We here show that in all settings, the images obtained by conventional polarised light microscopy and using the Glissando POL scanner were comparable. We conclude that the Glissando POL scanner can be safely integrated into a digital pathology workflow.