FolSas2 is a regulator of early effector gene expression during Fusarium oxysporum infection

Fusarium oxysporum f. sp. lycopersici (Fol) that causes a globally devastating wilt disease on tomato relies on the secretion of numerous effectors to mount an infection, but how the pathogenic fungus precisely regulates expression of effector genes during plant invasion remains elusive. Here, using molecular and cellular approaches, we show that the histone H4K8 acetyltransferase FolSas2 is a transcriptional regulator of early effector gene expression in Fol. Autoacetylation of FolSas2 on K269 represses K335 ubiquitination, preventing its degradation by the 26S proteasome. During the early infection process, Fol elevates FolSas2 acetylation by differentially changing transcription of itself and the FolSir1 deacetylase, leading to specific accumulation of the enzyme at this stage. FolSas2 subsequently activates the expression of an array of effectors genes, and as a consequence, Fol invades tomato successfully. These findings reveal a regulatory mechanism of effector gene expression via autoacetylation of a histone modifier during plant fungal invasion.