Related Experiment Video
Updated: Jun 5, 2025

A Porcine Heterotopic Heart Transplantation Protocol for Delivery of Therapeutics to a Cardiac Allograft
Published on: February 14, 2022
Surgical techniques for cardiac allograft procurement and perfusion in controlled donation after circulatory death
Antonio Loforte1, Matteo Marro1, Erika Simonato1
1Department of Surgical Sciences, City of Health and Science University Hospital, Cardiac Surgery University Unit, University of Turin, Turin, Italy.
Abstract:
In the last decade, heart transplants using allografts from adult donors after circulatory death, in a controlled setting, controlled donation after circulatory death (cDCD) have been rapidly adopted and widely performed. The selection of retrieval methods has largely been determined by state or institutional guidelines concerning permissible postmortem procedures. A significant majority of cDCD heart recoveries have employed direct procurement and perfusion (DPP) followed by normothermic machine perfusion (NMP) for graft preservation. Another established method involves the thoracoabdominal normothermic regional perfusion (taNRP), which is then followed by either NMP or static cold storage. Processing, management and surgical techniques of heart allograft procurement after cDCD are herein described and discussed.

