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Related Experiment Videos

Pyruvate kinase in normal human thyroid tissue and thyroid neoplasms.

J N Verhagen, M C van der Heijden, J de Jong-van Dijken

    Cancer
    |January 1, 1985
    PubMed
    Summary

    Pyruvate kinase activity is significantly elevated in human thyroid carcinomas compared to normal tissue. This enzyme

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    Area of Science:

    • Biochemistry
    • Oncology
    • Molecular Biology

    Background:

    • Pyruvate kinase (ATP: pyruvate-2-O-phosphotransferase, EC 2.7.1.40) is a key enzyme in glycolysis.
    • Altered enzyme activity is often observed in various cancers, including thyroid malignancies.

    Purpose of the Study:

    • To investigate pyruvate kinase activity and isoenzyme composition in human thyroid carcinomas, follicular adenomas, and normal thyroid tissue.
    • To correlate enzyme activity and characteristics with tumor type and proliferative activity.

    Main Methods:

    • Enzyme-specific activity assays were performed on tissue samples.
    • Isoenzyme composition was analyzed using electrophoresis.
    • Kinetic properties and inhibition patterns were examined.

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    Main Results:

    • Specific pyruvate kinase activity was significantly higher in thyroid carcinomas (0.94 +/- 0.44) than in normal thyroid tissue (0.14 +/- 0.05) (P < 0.0001).
    • Follicular adenomas showed heterogeneous pyruvate kinase activity, with higher levels correlating with increased proliferative activity.
    • Carcinomas predominantly expressed K4 and K3M pyruvate kinase isoenzymes, with undifferentiated tumors showing more K4 type.
    • Malignant tissues exhibited increased inhibition of pyruvate kinase by L-alanine compared to normal tissues, attributed to a higher proportion of K subunits.

    Conclusions:

    • Elevated pyruvate kinase activity and altered isoenzyme profiles are characteristic of human thyroid carcinomas.
    • Pyruvate kinase may serve as a potential biomarker for thyroid tumor progression and malignancy.
    • The observed changes in pyruvate kinase suggest its involvement in the altered metabolic landscape of thyroid cancer.