Screen-Printed Nanohybrid Palladium-Based Electrodes for Fast and Simple Determination of Estradiol in Livestock

Claudio Sabbatini Capella Lopes ¹, Francisco Walison Lima Silva ¹, Juliana Dos Santos Fernandes ¹

⁰Departamento de Química Analítica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-853, Brazil.

Acs Omega +

|

December 9, 2024

View abstract on PubMed

Summary

A new nonenzymatic biosensor using palladium nanoparticles and carbon dots on a screen-printed electrode offers a simple, sensitive method for detecting estradiol (E2) in livestock. This disposable sensor provides accurate reproductive hormone analysis for animal breeding systems.

Area Of Science

Electrochemistry
Nanomaterials Science
Animal Reproductive Biology

Background

Accurate estradiol (E2) determination is crucial for animal breeding systems.
Existing methods for E2 detection can be complex or invasive.
Development of simple, minimally invasive detection methods is needed.

Purpose Of The Study

To develop a novel nonenzymatic biosensor for simple and direct estradiol detection in livestock samples.
To utilize a nanohybrid material composed of palladium nanoparticles (PdNPs) and carbon dots (C.dots) on a silica support for enhanced sensor performance.

Main Methods

Fabrication of a screen-printed electrode (SPE) modified with PdNPs/C.dots/SiO2 nanohybrids.
Characterization of nanomaterials using atomic force microscopy, high-resolution transmission electron microscopy, and electrochemical impedance.
Electrochemical detection of estradiol (E2) using the developed nonenzymatic biosensor.

Main Results

The SPE/PdNPs/C.dots/SiO2 biosensor demonstrated superior electrocatalytic activity for E2 oxidation compared to unmodified sensors.
High sensitivity was achieved with a linear detection range from 0.005 to 14.0 μmol L-1 and a limit of detection of 1.0 nmol L-1.
Accurate E2 recovery (92-106%) in bovine serum and urine samples, with minimal interference (<2%) from other substances.

Conclusions

The developed nonenzymatic biosensor offers a highly sensitive, selective, and practical approach for E2 determination in livestock.
The sensor's simple production, disposability, and high performance make it suitable for routine analysis in animal breeding.
This technology facilitates efficient monitoring of reproductive hormones, supporting improved animal health and breeding outcomes.