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Updated: Jun 5, 2025

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
Published on: June 21, 2018
Rapid detection of volatile organic compounds emitted from plants by multicapillary column-ion mobility spectrometry
Vahideh Ilbeigi1, Younes Valadbeigi2, Martina Zvaríková3
1Department of Experimental Physics, Comenius University, Mlynská dolina F2, 84248 Bratislava, Slovakia. vahideh.ilbeigi@fmph.uniba.sk.
Abstract:
This study presents a novel rapid analytical method for the detection of volatile organic compounds (VOCs) emitted from blueberry leaves using the Tenax adsorbent followed by separation using a multicapillary column (MCC) and Ion Mobility Spectrometry (IMS) detection. The emitted VOCs including caryophyllene, benzene acetonitrile, linalool, ocimene, and methyl salicylate initiated by different stress factors including mechanical damage (punching), herbivore attack (aphids) and methyl jasmonate (MeJA) spraying were detected and quantified. Limits of Detection (LODs) for the VOCs were determined in the range of 8 to 33 ng. This new cost-efficient method provided a simple and direct detection of the emitted VOCs from plants without any sample pretreatment.
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