Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

915
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
915

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cuprorivaite as a Multifunctional Material for Hypertrophic Scar Modulation with Intrinsic Photothermal Enhancement.

ACS applied materials & interfaces·2026
Same author

Lipid trapping slows ball-and-chain inactivation in a calcium-activated potassium channel.

Nature communications·2026
Same author

Structural basis for activation and potentiation in a human α5β3 GABA<sub>A</sub> receptor.

Nature communications·2026
Same author

U-ResNet-ESPI: a physics-informed deep learning framework for robust phase unwrapping in electronic speckle pattern interferometry.

Optics express·2026
Same author

Hydrophobic and lipid-mediated gating mechanism revealed by low-conductance MthK mutants.

bioRxiv : the preprint server for biology·2026
Same author

Comprehensive network pharmacology study on Huangjing Wan in the treatment of ischaemic stroke.

Medicine·2026

Related Experiment Video

Updated: May 5, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

8.9K

Refractive Index Morphology Imaging Microscope System Utilizing Polarization Multiplexing for Label-Free Single

Huijun Wang1,2, Lu Zhang1,2, Chen Fan1,2

  • 1State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an 710049, China.

ACS Sensors
|December 9, 2024
PubMed
Summary

A new microscope system (RIMIM) can quickly separate cell refractive index and morphology without labels. This breakthrough aids in detecting diseases like cancer by analyzing live cell structures and internal substances.

Keywords:
decoupleimaging microscopelabel-freemorphologyphasepolarizationrefractive indexsingle living cellsthickness

More Related Videos

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

8.4K
Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy
05:54

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Published on: September 8, 2023

1.1K

Related Experiment Videos

Last Updated: May 5, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

8.9K
Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

8.4K
Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy
05:54

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Published on: September 8, 2023

1.1K

Area of Science:

  • Biophysics
  • Cell Biology
  • Optical Imaging

Background:

  • Label-free detection of internal cell substances and morphologies is vital for diagnosing diseases.
  • Current methods for decoupling refractive index (RI) and morphology are complex and slow.
  • A need exists for simple, rapid techniques to analyze live cell characteristics.

Purpose of the Study:

  • To introduce a novel refractive index morphology imaging microscope (RIMIM) system.
  • To enable simultaneous, label-free decoupling of intracellular refractive index distribution (IRID) and cell morphology.
  • To demonstrate the system's utility in cytopathology detection.

Main Methods:

  • Utilized polarization multiplexing for simultaneous degree of circular polarization (DOCP) and noninterferometric quantitative phase imaging (QPI).
  • Employed a circular depolarization decay model (CDDM) to retrieve IRID from optical thickness and DOCP data.
  • Decoupled cell thickness from phase results using the retrieved IRID.

Main Results:

  • Successfully retrieved IRID and decoupled fine morphology in mouse forestomach carcinoma (MFC) and human kidney-2 (HK-2) cells.
  • Demonstrated effective detection of membrane damage and morphological changes in ferroptotic HK-2 cells.
  • Observed significant increases in surface fold measurements for damaged cells compared to normal cells.

Conclusions:

  • The RIMIM system offers a simple and rapid method for label-free decoupling of RI and morphology in live cells.
  • This technique shows significant potential for advancing cytopathology and disease detection.
  • The RIMIM system provides a valuable tool for real-time analysis of cellular health and disease states.