Topography immune-responsive silk films for skin regeneration

Scar formation and chronic refractory wounds pose a significant threat to public health, with abnormal immune regulation as a key characteristic. However, topography, a crucial factor influencing immune responses, has not been adequately considered in the design of wound dressings. In this study, we constructed a hierarchical structure on silk fibroin (SF) films by combining soft lithography and femtosecond laser ablation, without altering the intrinsic properties of SF. The discontinuity in the hierarchical structure induced a transformation in the morphology of macrophage RAW264.7 cells from round to spindle or pancake-like shapes, leading to phenotypic polarization toward M2 or M1. The timely transition from M1 to M2 polarization and the balance between these states promoted fibroblast L929 cells to express mRNA for FN, coll-I, TGF-β1, and α-SMA. The hierarchical structure of SF films facilitates full-thickness wound repair in vivo by regulating inflammation and promoting neovascularization and collagen deposition. Thus, hierarchical topography presents a promising strategy for the design of immunomodulatory wound dressings.