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Related Experiment Video

Updated: Jan 30, 2026

A Two-Step Method for Percutaneous Transhepatic Choledochoscopic Lithotomy
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Human monocyte-derived mucus secretagogue.

Z Marom, J H Shelhamer, M Kaliner

    The Journal of Clinical Investigation
    |January 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Stimulated human monocytes release a factor, monocyte-derived mucus secretagogue (MMS), that prompts airway mucus secretion. This molecule is similar to one released by pulmonary macrophages.

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    Area of Science:

    • Immunology
    • Respiratory Medicine
    • Cell Biology

    Background:

    • Human pulmonary macrophages release factors that stimulate airway mucus secretion.
    • The role of peripheral monocytes in mucus secretion is less understood.

    Purpose of the Study:

    • To investigate if human peripheral monocytes release factors that stimulate mucous glycoprotein release from human airways.
    • To characterize the nature of this secreted factor.

    Main Methods:

    • Human peripheral monocytes were stimulated with opsonized zymosan or Staphylococcus aureus.
    • Supernatants were analyzed for their ability to stimulate mucus release from cultured human airways.
    • Chromatographic and isoelectric focusing techniques were used to characterize the active factor.

    Main Results:

    • Monocyte supernatants stimulated mucus secretion from human airways.
    • The active factor, termed monocyte-derived mucus secretagogue (MMS), was found in supernatants from activated monocytes.
    • MMS has a molecular weight of approximately 2,000 and an isoelectric point (pI) of 5.2.
    • MMS is a newly synthesized molecule, not detected in cell lysates or formed in the presence of cycloheximide.
    • MMS is not a prostaglandin or eicosanoid.

    Conclusions:

    • Stimulated human peripheral monocytes synthesize and release a small, acidic molecule (MMS).
    • MMS stimulates mucus secretion in human airways.
    • The properties of monocyte-derived MMS are nearly identical to pulmonary macrophage-derived MMS.