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Related Experiment Videos

Variable radiosensitivity in fibroblasts from patients with tuberous sclerosis.

A Hayashi, Y Yoshida, H Tanaka

    The Journal of Investigative Dermatology
    |January 1, 1985
    PubMed
    Summary

    Cultured cells from tuberous sclerosis (TS) patients generally show normal x-ray sensitivity. However, some tumor-derived cells and previously identified hypersensitive strains exhibit increased sensitivity, suggesting acquired radiosensitivity in TS cells.

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    Area of Science:

    • Cell Biology
    • Genetics
    • Radiation Oncology

    Background:

    • Tuberous sclerosis (TS) is a genetic disorder.
    • Previous studies indicated hypersensitivity to radiation in some TS cell lines.
    • The radiosensitivity of TS-derived fibroblasts requires further investigation.

    Purpose of the Study:

    • To evaluate the x-ray sensitivity of cultured fibroblast cell strains from tuberous sclerosis patients.
    • To determine if cell strains from tumorous skin exhibit altered radiosensitivity.
    • To correlate x-ray sensitivity with previously observed gamma-ray hypersensitivity.

    Main Methods:

    • Cultured fibroblast cell strains from 11 TS patients were established.
    • Colony-forming ability was assessed after x-ray irradiation.

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  • Sensitivity was compared between cells from normal-appearing skin and tumorous skin.
  • Comparison with normal control cell strains was performed.

    • Main Results:

    • Fibroblast cell strains from normal-appearing skin of TS patients showed normal x-ray sensitivity.
    • Cell strains from tumorous skin did not exhibit hypersensitivity.
    • A trend towards lower D0/D10 values was observed in tumorous skin strains.
    • Hypersensitivity to x-rays was confirmed in TS cell strains previously sensitive to gamma-rays.

    Conclusions:

    • Cultured cells from tuberous sclerosis patients generally display normal x-ray sensitivity.
    • Some TS cell lines may acquire hypersensitivity under specific conditions.
    • Tumorigenic cells in TS might possess altered radiosensitivity profiles.