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Complete genome sequence of multidrug-resistant and NDM-producing Klebsiella variicola clinical isolates

Alejandro Alvarado-Delgado1, Neli Nava-Dominguez1, Nadia Rodriguez-Medina1

  • 1Instituto Nacional de Salud Pública (INSP), Centro de Investigación sobre Enfermedades Infecciosas (CISEI), Laboratorio de Resistencia Bacteriana, Cuernavaca, Morelos, Mexico.

Microbiology Resource Announcements
|December 10, 2024
PubMed

Insights

Global spread of NDM-producing Klebsiella variicola is increasing. This study performed whole-genome sequencing on clinical isolates to understand the multidrug-resistant and NDM-producing Klebsiella variicola.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • The emergence and global spread of carbapenemase-producing Enterobacteriaceae, particularly New Delhi metallo-beta-lactamase (NDM), pose a significant public health threat.
  • Klebsiella variicola is increasingly recognized as a reservoir and disseminator of antimicrobial resistance genes, including NDM.

Purpose of the Study:

  • To characterize the whole-genome sequences of NDM-producing Klebsiella variicola clinical isolates.
  • To investigate the genetic basis of multidrug resistance in these isolates.

Main Methods:

  • Whole-genome sequencing (WGS) was performed on clinical isolates of Klebsiella variicola.
  • Antimicrobial susceptibility testing was conducted.
  • Bioinformatic analysis was used to identify resistance genes and mobile genetic elements.

Main Results:

  • The study successfully sequenced the genomes of NDM-producing Klebsiella variicola clinical isolates.
  • Genomic analysis revealed the presence of the NDM gene and other resistance determinants.
  • The genetic context and mobile elements associated with NDM were elucidated.

Conclusions:

  • Whole-genome sequencing provides valuable insights into the genetic makeup of multidrug-resistant NDM-producing Klebsiella variicola.
  • Understanding the genomic epidemiology of these isolates is crucial for controlling their spread in healthcare and environmental settings.
Keywords:
Klebsiella pneumoniae species complexcarbapenem-resistantclassical phenotype

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