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Random Sanitization in DNA Information Storage Using CRISPR-Cas12a.

Hongyu Shen1, Zhi Weng1, Haipei Zhao1

  • 1School of Biomedical Engineering, The International Peace Maternity and Child Health Hospital, Zhangjiang Institute for Advanced Study and National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, China.

Journal of the American Chemical Society
|December 10, 2024
PubMed
Summary
This summary is machine-generated.

This study introduces random sanitization in DNA data storage using CRISPR-Cas12a (RSDISC). This method securely erases DNA files, achieving high efficiency and enabling secure data management.

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Area of Science:

  • Biotechnology
  • Genomics
  • Information Science

Background:

  • DNA data storage offers high density and stability for metadata.
  • Current DNA storage lacks secure methods for data wiping.

Purpose of the Study:

  • To develop a secure and efficient method for random sanitization in DNA information storage.
  • To enable selective file erasure and enhance data security in DNA storage systems.

Main Methods:

  • Utilized CRISPR-Cas12a's collateral cleavage of single-stranded DNA (ssDNA).
  • Controlled primer-template hybridization thermodynamics for selective ssDNA degradation.
  • Applied random sanitization in DNA information storage using CRISPR-Cas12a (RSDISC).

Main Results:

  • Achieved up to 99.9% sanitization efficiency for 28,258 oligonucleotides in one round.
  • Demonstrated potential for erasing up to 10^12 files based on hybridization efficiency models.
  • Showcased selective degradation of ssDNA with varying GC content, length, and structure.

Conclusions:

  • RSDISC provides a robust random sanitization method for DNA data storage.
  • This approach supports information encryption, file classification, and memory deallocation.
  • Establishes a foundation for secure and accurate data handling in DNA storage.