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Updated: Jun 5, 2025
Protocols for Testing the Toxicity of Novel Insecticidal Chemistries to Mosquitoes
Published on: February 13, 2019
DETERMINING A DIAGNOSTIC DOSE OF PIRIMIPHOS-METHYL FOR AEDES AEGYPTI USING TREATED BOTTLES1
Gabriela González-Olvera1, Rita L Vizcaino-Cabarrus2, Alicia Méndez-Manzanero1
1Collaborative Unit for Entomological Bioassays, Campus de Ciencias Biologicas y Agropecuarias, Universidad Autónoma de Yucatán, Mérida, Yucatan, Mexico. Carretera Mérida-Xmatkuil Km. 15.5 Apdo., C.P. 97100.
Abstract:
There is a pressing need for innovative strategies to control arboviruses transmitted by Aedes aegypti. The modification of indoor residual spraying to target Ae. aegypti is one such strategy. A clinical trial quantifying the epidemiologic impact of targeted indoor residual spraying for Ae. aegypti control used a product with pirimiphos-methyl as the active ingredient in the city of Mérida, Mexico. To monitor the susceptibility of local Ae. aegypti populations over the course of the trial, we calculated a diagnostic dose for pirimiphos-methyl using the Centers for Disease Control and Prevention bottle assay. Two independent laboratories tested a series of 8 concentrations of pirimiphos-methyl, eliciting a range of mortality between 0% and 100% in an insecticide-susceptible reference strain of Ae. aegypti. The results suggested a diagnostic dose of 25 μg/ml at a diagnostic time of 30 min. This diagnostic dose of pirimiphos-methyl was used to monitor pirimphos-methyl susceptibility in Ae. aegypti throughout the trial.
Insights
Researchers established a diagnostic dose of 25 μg/ml for pirimiphos-methyl to monitor insecticide resistance in Aedes aegypti mosquitoes during a clinical trial in Mexico. This helps track mosquito control effectiveness.
Area of Science:
- Medical Entomology
- Vector-Borne Disease Control
- Insecticide Resistance
Background:
- Arboviruses transmitted by Aedes aegypti pose a significant public health threat, necessitating innovative control strategies.
- Indoor residual spraying (IRS) is a key vector control method, with modifications targeting Ae. aegypti showing promise.
- A clinical trial in Mérida, Mexico, evaluated the epidemiologic impact of targeted IRS for Ae. aegypti control using pirimiphos-methyl.
Purpose of the Study:
- To establish a diagnostic dose for pirimiphos-methyl to monitor susceptibility in local Ae. aegypti populations.
- To assess the practical application of this diagnostic dose throughout a clinical trial.
Main Methods:
- The Centers for Disease Control and Prevention (CDC) bottle assay was employed to determine the diagnostic dose.
- Two independent laboratories tested eight concentrations of pirimiphos-methyl against a susceptible Ae. aegypti reference strain.
- Mortality rates were recorded to establish the dose-response relationship.
Main Results:
- A diagnostic dose of 25 μg/ml pirimiphos-methyl was determined.
- A diagnostic time of 30 minutes was established for the assay.
- This dose was subsequently used to monitor Ae. aegypti susceptibility during the clinical trial.
Conclusions:
- A reliable diagnostic dose of pirimiphos-methyl (25 μg/ml) was established for monitoring Ae. aegypti susceptibility.
- This method provides a crucial tool for evaluating the efficacy of IRS programs and managing insecticide resistance.
- The established dose supports ongoing vector control efforts against arboviruses in Mérida, Mexico.
