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OoTrap: enhancing oocyte collection and maturation with a field-deployable fluidic device.

Roksan Franko1,2, Marcia de Almeida Monteiro Melo Ferraz1,2

  • 1Clinic of Ruminants, Faculty of Veterinary Medicine, Ludwig-Maximilians-Universität München, Sonnenstr. 16, Oberschleißheim, 85764, Germany. m.ferraz@lmu.de.

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Summary

OoTrap streamlines assisted reproductive technologies (ART) by reducing stress and improving oocyte quality. This novel device enhances in vitro maturation (IVM) and is suitable for field applications.

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Area of Science:

  • Reproductive biology
  • Biotechnology
  • Animal conservation

Background:

  • Manual handling in assisted reproductive technologies (ART) can negatively impact oocyte and embryo quality.
  • Conventional ART methods often lack the mechanical forces present in the natural in vivo environment.

Purpose of the Study:

  • To introduce OoTrap, a novel fluidic device designed to streamline ART workflows.
  • To improve oocyte capture, maturation, and overall quality in ART.
  • To provide a controlled microenvironment that mimics in vivo conditions and enables field applications.

Main Methods:

  • OoTrap device facilitates oocyte capture and maturation in a compact unit.
  • Device operates in static and perfusion modes, reintroducing mechanical forces.
  • Incorporates a heating system and 3D-printed syringe pump for ex-incubator operation.

Main Results:

  • OoTrap achieved higher in vitro maturation (IVM) rates under perfusion.
  • Oocytes matured with OoTrap exhibited fewer chromosomal abnormalities.
  • Spindle morphology integrity was maintained, and device is suitable for field applications.

Conclusions:

  • OoTrap enhances ART outcomes by minimizing manual handling and providing a controlled microenvironment.
  • The device offers a practical solution for field-based ART applications, improving efficiency and oocyte quality.
  • OoTrap has the potential to advance both reproductive medicine and species conservation efforts.