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Low cost, portable, 3D printable tissue precision slicer.

Beatriz Martinez-Martin¹, Isabella Lambros², Lukas Nuesslein²

¹Molecular and Cellular Biology Graduate Program, University of Massachusetts Amherst, Amherst, MA 01003, United States.

|December 11, 2024

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Researchers developed a new, affordable 3D-printed slicer for live tissue samples. This portable device enables precise sectioning of tissues, like brain organoids, for improved cell culture and analysis.

Area of Science:

Biotechnology
Histology
Stem Cell Research

Background:

Tissue slicing is crucial for histology and organotypic culture.
Existing devices like cryostats and vibratomes are often expensive, bulky, or unsuitable for live tissue slicing.
There is a need for accessible, precise, and sterile tissue sectioning methods for live samples.

Purpose of the Study:

To develop and validate a cost-effective, portable device for slicing live tissues.
To ensure the device is suitable for sterile laboratory environments and subsequent tissue culture.
To assess the precision, accuracy, and biological impact of the device on tissue samples.

Main Methods:

Designed and fabricated a novel tissue slicer using 3D-printed Nylon-12 for autoclave sterilization.

Keywords:

Low-cost Microtomy Organoids Tissue slices

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Evaluated the device's portability and suitability for use within a biosafety cabinet.

Sectioned human pluripotent stem cell-derived brain organoids and performed subsequent long-term tissue culture.

Main Results:

The 3D-printed device achieved precise and accurate slicing of brain organoids.
Sliced organoid tissues remained viable after extended culture periods.
Organoid slices exhibited an improved proliferation rate compared to unsliced controls.

Conclusions:

The developed 3D-printed tissue slicer offers a cost-effective and practical solution for live tissue sectioning.
The device facilitates sterile handling and supports the viability and proliferation of cultured tissue slices.
This innovation can advance research in histology, organoid culture, and regenerative medicine.