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A Universal Protocol for Large-scale gRNA Library Production from any DNA Source
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A Universal Protocol for Large-scale gRNA Library Production from any DNA Source

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Protocol for iterative enrichment of integrated sgRNAs via derivative CRISPR-Cas9 libraries from genomic DNA of

Adriana Ordóñez1, David Ron2, Heather P Harding2

  • 1Cambridge Institute for Medical Research (CIMR), University of Cambridge, Cambridge Biomedical Campus, The Keith Peters Building, Cambridge CB2 0XY, UK; Universidad Católica de Murcia (UCAM), UCAM HiTech, Campus de los Jerónimos 135, Guadalupe E-30107, Spain.

STAR Protocols
|December 11, 2024
PubMed

Keywords:
CRISPRCell BiologyHigh-Throughput ScreeningMicroscopyMolecular Biology

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