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Related Experiment Video

Updated: Jun 5, 2025

Tractable Mammalian Cell Infections with Protozoan-primed Bacteria

Tractable Mammalian Cell Infections with Protozoan-primed Bacteria

Published on: April 2, 2013

Legionella uses host Rab GTPases and BAP31 to create a unique ER niche.

Attinder Chadha¹, Yu Yanai², Hiromu Oide²

¹G.W. Hooper Foundation, University of California, San Francisco, San Francisco, CA, USA; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA.

|December 11, 2024

Summary

Legionella pneumophila manipulates host endoplasmic reticulum (ER) vesicles to form a vacuole. This study reveals a transition from smooth ER association to a distinct rough ER niche, crucial for bacterial growth.

Keywords:

CP: Cell biology CP: Microbiology Legionella Rab GTPase bacteria endoplasmic reticulum pathogen

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Area of Science:

Cell Biology
Microbiology
Infectious Diseases

Background:

Legionella pneumophila forms a unique vacuole (LCV) using host endoplasmic reticulum (ER) vesicles.
The precise relationship between the LCV membrane and the host ER network remains incompletely understood.

Purpose of the Study:

To investigate the dynamic association of the LCV with the host ER network during Legionella infection.
To identify host and bacterial factors involved in the LCV's membrane remodeling process.

Main Methods:

Microscopy to visualize LCV association with smooth ER (sER) and rough ER (rER).
Genetic manipulation of host factors (Rab10, Rab4, BAP31) and Legionella effector (Lpg1152).
Analysis of bacterial growth under different host-factor conditions.

Main Results:

Early infection shows LCV association with host sER.
Later stages reveal a distinct rER niche for Legionella, disconnected from the host ER.
Host proteins Rab10, Rab4, and BAP31 are essential for the sER to rER transition.
Legionella effector Lpg1152 binds BAP31, and both are critical for optimal bacterial growth.

Conclusions:

The LCV membrane undergoes a significant transformation from an sER-associated structure to a distinct rER niche.
Host factors (Rab GTPases, BAP31) and a Legionella effector (Lpg1152) orchestrate this dynamic membrane remodeling.
This complex host-pathogen interaction is vital for Legionella's intracellular replication.