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Updated: Jun 5, 2025
LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles
Published on: February 1, 2020
Cytogenomic Characterization of Murine Osteosarcoma Cell Line SEWA
Thomas Liehr1, Martina Rincic2
1Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Jena, Germany.
Introduction:
The SEWA cell line, which is derived from a virus-induced murine osteosarcoma (OS) ascites, was established in the 1980s from a serially transplanted male-derived tumor that was first published in 1961. It has been applied in about 50 studies but was never genetically characterized in detail; this study fills that gap.
Methods:
The SEWA cell line was analyzed for its chromosomal constitution using molecular cytogenetic approaches. Array comparative genomic hybridization was performed to characterize copy number alterations.
Results:
SEWA has a near-diploid karyotype without Y-chromosome material. The complex karyotype includes neocentrics and simple and complex rearrangements. Amplification of MYC oncogene was detected in two homogeneously staining regions on two different derivative chromosomes.
Conclusion:
An in silico translation of the obtained results to the human genome indicated that SEWA is suitable as a model for advanced human OS.
