Adapting Caenorhabditis elegans to evaluating functional foods and ingredients for cholesterol absorption

The nematode Caenorhabditis elegans is an excellent model organism for elucidating higher life phenomena. C. elegans and humans are common in many aspects. During our research on development and life span regulation, we identified RAB-18, a small guanosine triphosphatase (GTPase) involved in the membrane trafficking of NCR-1, a cholesterol transporter mainly expressed in the intestine. We expressed the human NPC1L1, an intestinal cholesterol transporter, in mutant C. elegans lacking NCR-1. NPC1L1-expressing animals revealed almost the same larval diapause in the presence of a diapause-inducing pheromone and lipid droplets containing cholesterol as in wild-type C. elegans. This result indicates that C. elegans NCR-1 and human NPC1L1 are exchangeable and that C. elegans RAB-18 transports human NPC1L1 to the apical membrane in the C. elegans intestine. This transgenic C. elegans could be adapted to evaluate functional foods and ingredients regarding cholesterol absorption.