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Lea blood group antigen on human platelets.

R A Dunstan, M B Simpson, W F Rosse

    American Journal of Clinical Pathology
    |January 1, 1985
    PubMed
    Summary

    Human platelets possess the Lewis a (Lea) antigen, confirmed by radioligand assays. Platelets can also adsorb Lea antigen from plasma, similar to red blood cells.

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    Area of Science:

    • Immunology
    • Hematology
    • Biochemistry

    Background:

    • The presence of Lewis antigens on human blood cells is well-established for red blood cells.
    • The expression of Lewis antigens on platelets has not been definitively characterized.

    Purpose of the Study:

    • To investigate the presence of the Lewis a (Lea) antigen on human platelets.
    • To determine if platelets can acquire Lea antigen from plasma.

    Main Methods:

    • Utilized one- and two-stage radioligand assays with purified anti-Lea antibodies (goat and human IgG).
    • Employed affinity chromatography, radioiodination (125I), and Staph protein A chromatography for antibody preparation.
    • Assayed platelet-bound antigen using gamma scintillation counting after centrifugation through phthalate esters.

    Main Results:

    • Demonstrated the presence of Lea antigen on platelets from individuals with Lea+ red blood cells using dose response and saturation curve analyses.
    • Observed that platelets from an Le(a-b-) donor adsorbed Lea antigen when incubated in Le (a+b-) plasma, mirroring red blood cell behavior.

    Conclusions:

    • Human platelets express the Lewis a antigen.
    • Platelets exhibit the capacity to adsorb Lea antigen from plasma, similar to red blood cells.
    • The clinical relevance of Lea antigen on platelets in transfusion medicine requires further investigation.

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