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Related Concept Videos

Viral Replication: Lysogenic Cycle01:16

Viral Replication: Lysogenic Cycle

The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects...
Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

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Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
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Lysogenic Cycle of Bacteriophages00:43

Lysogenic Cycle of Bacteriophages

61.8K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
61.8K
Viral Replication: Lytic Cycle01:20

Viral Replication: Lytic Cycle

1
Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
1
Antibiotic Selection00:57

Antibiotic Selection

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Overview
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Related Experiment Video

Updated: Jun 5, 2025

Following Cell-fate in E. coli After Infection by Phage Lambda
06:10

Following Cell-fate in E. coli After Infection by Phage Lambda

Published on: October 14, 2011

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Research on phage λ: a lucky choice.

Dale E A Lewis1, Sankar Adhya1

  • 1Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.

Ecosal Plus
|December 12, 2024
PubMed
Summary

Bacteriophage lambda (λ) uses a genetic switch to control its lytic or lysogenic lifestyles. The CI repressor

Area of Science:

  • Molecular Biology
  • Genetics
  • Microbiology

Background:

  • Bacteriophage lambda (λ) is a model organism for studying gene regulation.
  • The phage's genetic switch governs its transition between lytic and lysogenic cycles.
  • Understanding these mechanisms provides fundamental insights into molecular biology.

Purpose of the Study:

  • To detail the molecular mechanisms of bacteriophage λ lysogeny.
  • To explain the function of the genetic switch in phage λ.
  • To elucidate the role of the CI repressor in regulating phage lifestyles.

Main Methods:

  • Review of existing literature on bacteriophage λ.
  • Analysis of genetic regulatory networks.
  • Description of molecular interactions involved in lysogeny.
Keywords:
DNA loopingactivatoroperatorpromoterrepressor

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Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics
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Author Spotlight: Efficiently Eliminating Bacteriophages from Infected Salmonella Cultures Using Lipopolysaccharides
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Main Results:

  • The CI repressor plays a central role in establishing and maintaining lysogeny.
  • Cooperative binding of CI repressor to operators controls phage gene expression.
  • Autoregulation by CI repressor ensures repression of lytic promoters and activation of lysogenic promoters.

Conclusions:

  • Bacteriophage λ lysogeny is a well-defined genetic regulatory process.
  • The study of phage λ has significantly advanced our understanding of gene expression.
  • Current understanding details the molecular basis of λ's dual lifestyle decision.