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Lipase secretion from dispersed rabbit gastric glands
The American Journal of Physiology
|January 1, 1985
Summary
Rabbit gastric glands secrete lipase, an enzyme crucial for triglyceride digestion. Secretion is stimulated by cholecystokinin octapeptide (CCK-8) and carbachol via distinct receptor pathways, requiring energy for release.
Area of Science:
- Gastroenterology
- Enzymology
- Cell Biology
Background:
- Gastric lipase plays a role in lipid digestion.
- Understanding gastric lipase secretion mechanisms is important for digestive physiology.
Purpose of the Study:
- To investigate the secretion of gastric lipase from isolated rabbit gastric glands.
- To identify the signaling pathways and energy requirements involved in gastric lipase release.
Main Methods:
- Gastric lipase activity was measured by quantifying tri[3H]olein hydrolysis in dispersed rabbit gastric glands.
- The effects of secretagogues (CCK-8, carbachol, histamine) and inhibitors (carbonyl cyanide m-chlorophenylhydrazone, dibutyryl cGMP, atropine) on lipase secretion were assessed.
Main Results:
- Basal lipase release was low (1.5-4.5%).
- CCK-8 and carbachol significantly stimulated lipase secretion in a dose-dependent manner.
- Lipase secretion is dependent on mitochondrial energy production and involves distinct receptor-mediated pathways.
Conclusions:
- Rabbit stomach mucosa secretes a lipase capable of hydrolyzing long-chain triglycerides.
- Gastric lipase secretion is regulated by at least two distinct receptor mechanisms, modulated by energy availability.