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Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Complete genome sequence analysis of a novel nepovirus isolated from Jasminum polyanthum in Yunnan, China
Xiyan Zhao1, Rex Frimpong Anane2, Jianwei Guo3
1Key Laboratory for Agricultural Bioaffiliationersity for Pest Management of China, Ministry of Education, Yunnan Agricultural University, NO. 95, Jinhei Road, Panlong District, Kunming, Yunnan, 650201, China.
Abstract:
Here, we report the complete genome sequence of Jasminum polyanthum nepovirus 1 (JPV1), a novel virus associated with foliar ringspot symptoms in Jasminum polyanthum (Oleaceae). The genome of JPV1 consists of RNA1 and RNA2, both of which are monocistronic and are 7081 nt and 4212 nt in length, respectively. RNA1 encodes six functional proteins, including the X1 protein, X2 protein, NTP-binding protein (NTB), VPg protein, protease cofactor (Pro), and RNA-dependent RNA polymerase (RdRp), while RNA2 encodes three functional proteins: the 2A protein, movement protein (MP), and capsid protein (CP). Pairwise sequence comparisons showed that the JPV1 protease-polymerase (Pro-Pol) and CP proteins each shared less than 40% aa sequence identity with those of other nepoviruses. Phylogenetic analysis indicated that JPV1 is a putative member of the genus Nepovirus. Based on these results, we propose that JPV1 is a member of subgroup B of the genus Nepovirus.
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