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Updated: Jun 13, 2025
Murine Superficial Lymph Node Surgery
Published on: May 21, 2012
Protocol for in situ and in vivo dissection of mouse lung-draining lymph nodes
Enshuo Zhang1, Baicheng Zhao1, Yupeng Liu2
1Department of Thoracic Surgery, Shanghai Chest Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China.
Lung-draining lymph nodes (LNs) play crucial roles in infection, inflammation, and cancer, but in situ and in vivo studying models are technically challenging. Here, we present a protocol for the in situ and in vivo dissection of mouse lung-draining LNs, ensuring postoperative survival. We describe steps for lymphadenectomy including localizing lung-draining LNs, open thoracotomy, and LN dissection. We then detail procedures for surgical sample preservation and postoperative care. This protocol offers broad applications in immunological and oncological research.
Lung-draining lymph nodes (LNs) play crucial roles in infection, inflammation, and cancer, but in situ and in vivo studying models are technically challenging. Here, we present a protocol for the in situ and in vivo dissection of mouse lung-draining LNs, ensuring postoperative survival. We describe steps for lymphadenectomy including localizing lung-draining LNs, open thoracotomy, and LN dissection. We then detail procedures for surgical sample preservation and postoperative care. This protocol offers broad applications in immunological and oncological research.
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