Protocol for in situ and in vivo dissection of mouse lung-draining lymph nodes

Lung-draining lymph nodes (LNs) play crucial roles in infection, inflammation, and cancer, but in situ and in vivo studying models are technically challenging. Here, we present a protocol for the in situ and in vivo dissection of mouse lung-draining LNs, ensuring postoperative survival. We describe steps for lymphadenectomy including localizing lung-draining LNs, open thoracotomy, and LN dissection. We then detail procedures for surgical sample preservation and postoperative care. This protocol offers broad applications in immunological and oncological research.