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Physiological consequences of Aldolase C deficiency during lactation
James A Votava1,2, Jing Fan2,3, Brian W Parks1
1Department of Nutritional Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States of America.
Plos One
|December 12, 2024
Summary
Aldolase C (Aldoc) is crucial for mammary gland function during lactation. Its absence in Aldoc knockout mice significantly impacts milk composition and pup development, highlighting Aldoc's role in milk synthesis.
Area of Science:
- Biochemistry
- Molecular Biology
- Reproductive Biology
Background:
- The lactating mammary gland requires de novo lipogenesis (DNL) for milk synthesis, utilizing glucose as a key substrate.
- Aldolase C (Aldoc), a glycolytic enzyme, is upregulated during lactation and is a target of Srebp1/2, regulators of DNL.
- Aldoc's specific role in mammary gland function and lactation remains largely uncharacterized.
Purpose of the Study:
- To investigate the functional role of Aldolase C (Aldoc) in the lactating mammary gland.
- To determine the impact of Aldoc deficiency on milk composition and offspring development.
Main Methods:
- Utilized whole-body Aldoc knockout (Aldoc-/-) mouse models.
- Analyzed pup body weight and milk biochemical composition (galactose, lactose, cholesterol).
- Performed mass spectrometry to analyze milk lipid profiles, specifically triglyceride content.
Main Results:
- Aldoc-/- dams exhibited reduced pup body weight, indicating impaired lactation.
- Milk from Aldoc-/- dams showed significantly higher galactose and lower lactose and cholesterol levels.
- Milk lipids from Aldoc-/- dams contained significantly lower quantities of medium and long-chain fatty acid triglycerides.
Conclusions:
- Aldolase C (Aldoc) plays a significant role in mammary gland lactose and lipid synthesis during lactation.
- Aldoc deficiency impacts milk quality, affecting offspring growth and development.
- These findings provide functional evidence for Aldoc's contribution to milk production in mammals.
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