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Updated: May 7, 2026
Optimized Analysis of In Vivo and In Vitro Hepatic Steatosis
Published on: March 11, 2017
Asiaticoside alleviated NAFLD by activating Nrf2 and inhibiting the NF-κB pathway
Yunfei Wei1, Yibo Zhang1, Baihe Zhan1
1State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
Background:
Nonalcoholic fatty liver disease (NAFLD) is a globally prevalent clinical problem of chronic liver disorder whose main pathogenic mechanisms are hepatic lipid accumulation, oxidative stress, and inflammatory reactions. Asiaticoside (Asi) is a compound derived from Centella asiatica, possesses antioxidant, antiphlogistic, antifibrotic, as well as wound healing properties.
Purpose:
The aim of this study was to investigate the effects of Asi on NAFLD and its potential mechanisms.
Study Design:
Two versions of experimental models were constructed using free fatty acids (FFAs)-stimulated HepG2 cells along with high-fat diet (HFD)-incited NAFLD in mice.
Methods:
The pivotal action of Nrf2 was then explored using Ml-385 or Nrf2-/- mice.
Results:
The results indicated that Asi activated the Nrf2 to alleviate oxidative stress, inhibited the NF-κB to reduce the inflammatory response, and notably decreased lipid droplets and alleviated steatosis.
Conclusion:
In conclusion, Asi demonstrates a potential to activate Nrf2 as well as inhibit the NF-κB pathway, there alleviate NAFLD.
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