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OsCYCBL1 and OsHTR702 positively regulate rice tolerance to cold stress
Chuan Ma1, Shiwei Zheng1, Songjin Yang1
1State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute of Sichuan Agricultural University of Rice Research Institute, Chengdu 611130, China.
Abstract:
Chaling wild rice (Oryza rufipogon Griff.) can survive winter due to its extreme cold tolerance, whereas cultivated rice (Oryza sativa L.) cannot. Here, we found that the expression level of OsCYCBL1 decreased relatively less at low temperatures in Chaling wild rice compared with cultivated rice. Transgenic assays of OsCYCBL1 in Nipponbare (Nip) showed that overexpression of OsCYCBL1 promoted cold tolerance. Transcriptome profiling, RT-qPCR analysis, and physiological parameters measurement indicated that overexpression of OsCYCBL1 maintained better DNA damage repair capacity, balanced the cell cycle, enhanced reactive oxygen species (ROS) homeostasis, and increased wax content, directly affecting the ICE-CBF-COR cascade. Moreover, OsHTR702, a gene that interacts with OsCYCBL1, also positively regulates rice cold tolerance by affecting the ICE-CBF-COR cascade and increasing ROS homeostasis at low temperatures. In addition, overexpression of OsCYCBL1 and OsHTR702 enabled rice to survive through winter. Taken together, the current results indicate that OsCYCBL1 and OsHTR702 are related to cold tolerance in rice, making them potential targets for enhancing crop resilience to cold stress.
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