Related Experiment Video
Updated: Jun 5, 2025
Multimodal Optical Microscopy Methods Reveal Polyp Tissue Morphology and Structure in Caribbean Reef Building Corals
Published on: September 5, 2014
Application of Optical Coherence Tomography (OCT) to evaluate the adverse effects of 2,4-D dimethylammonium on
Chutima Thanomsit1, Sugunya Kumla2, Jadsada Saetiew3
1Department of Fisheries, Faculty of Agriculture and Technology, Rajamangala University of Technology Isan Surin Campus, Surin 32000, Thailand.
Abstract:
2,4-D dimethylammonium, a widely used herbicide, accumulates in aquatic animals, posing risks to both their populations and human consumers. This study evaluated the effects of 2,4-D on Riceland prawn (Macrobrachium lanchesteri) using Optical Coherence Tomography (OCT) to assess morphological changes. The activity of acetylcholinesterase (AChE), catalase (CAT), and glutathione S-transferase (GST) was also measured. Toxicity increased with both concentration and exposure duration. OCT revealed swelling in the gill region, tissue abnormalities, and muscle damage in the abdominal somite, telson, and uropod, along with tissue separation and degradation. Histological analysis confirmed muscle degeneration, hemocyte infiltration, and swelling. AChE expression was undetectable at 400 and 450 µL/L after 96 hours, with a molecular weight of 71 kDa. CAT and GST activities decreased at higher herbicide concentrations, further demonstrating the harmful effects of 2,4-D on prawn health.
