Rapid start-up and metabolic evolution of partial denitrification/anammox process by hydroxylamine stimulation:
Chengkun Kao1, Qiong Zhang1, Jianwei Li1
1National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
Abstract:
Enhanced nitrogen removal by hydroxylamine (NH2OH) on anammox-related process recently received attention. This study investigated the impact of NH2OH on the partial-denitrification/anammox (PDA) biosystem. Results show that NH2OH (≤10 mg N/L) immediately induced nitrite accumulation and provided sufficient NO2- to anammox, achieving a 18.1 ± 4.3 % increase of nitrogen removal efficiency compared to the absence of NH2OH. Long-term exposure to NH2OH accelerated the functional microbial community transformation to PDA. Thauera was highly enriched (6.1 % → 26.9 %) along with Candidatus Brocadia increased in the biofilms, which mainly favor the coupling process of nitrate reduction and anammox. Although the migration mechanism of anammox and denitrifier revealed by CLSM-FISH alleviates the adverse effects of NH2OH, the anammox was inhibited when NH2OH exceeding 15 mg N/L through destroying the inner reduction of NO2-. These results suggested appropriate NH2OH addition favors the synergy between denitrifying and anammox bacteria, providing a promising option for wastewater treatment.
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