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Research on the effect of LAMP1 in the development and progression of ccRCC and its potential mechanism with LC3C-mediated autophagy

  • 0Department of Urology, Qilu Hospital of Shandong University, Jinan, Shandong, China.
Frontiers in Immunology +

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December 13, 2024

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December 13, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Low expression of Lysosome membrane-associated protein 1 (LAMP1) indicates poor prognosis in clear cell renal cell carcinoma (ccRCC). LAMP1 is a potential biomarker for ccRCC diagnosis and treatment.

Area Of Science

  • Oncology
  • Molecular Biology
  • Biochemistry

Background

  • Lysosome membrane-associated protein 1 (LAMP1) shows differential expression in tumors and influences tumor development.
  • Clear cell Renal Cell Carcinoma (ccRCC) is a prevalent renal cancer subtype with a poor prognosis.
  • The role and molecular mechanisms of LAMP1 in ccRCC remain largely unexplored.

Purpose Of The Study

  • To investigate the expression of LAMP1 in ccRCC and its clinical significance.
  • To elucidate the molecular mechanisms underlying LAMP1's function in ccRCC.
  • To evaluate LAMP1 as a potential diagnostic and prognostic biomarker for ccRCC.

Main Methods

  • Bioinformatic analysis of LAMP1 expression in ccRCC using various databases.
  • Western blot analysis of LAMP1 protein levels in ccRCC tissues and adjacent normal tissues.
  • Cell proliferation, invasion, and migration assays to assess LAMP1's functional impact.
  • Investigation of VHL and LC3C gene effects on LAMP1 expression.

Main Results

  • LAMP1 was significantly underexpressed in ccRCC, correlating with poorer prognosis (P<0.001).
  • Low LAMP1 expression was an independent prognostic marker for overall survival (P<0.05).
  • Elevated LAMP1 inhibited ccRCC cell proliferation, migration, and invasion; VHL(+) cells showed less LAMP1 expression than VHL(-) cells.

Conclusions

  • Reduced LAMP1 expression is linked to poor prognosis in ccRCC, positioning LAMP1 as a novel diagnostic and prognostic biomarker.
  • The study sheds light on LAMP1's potential mechanisms of action in renal cancer.
  • LAMP1 represents a promising therapeutic target for ccRCC treatment.

Keywords:

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