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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

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Updated: Jun 5, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

Developing, Characterizing, and Modeling CRISPR-Based Point-of-Use Pathogen Diagnostics.

Jaeyoung K Jung^1,2,3, Kathleen S Dreyer^1,2, Kate E Dray^1,2

¹Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois 60208, United States.

ACS Synthetic Biology

|December 13, 2024

Summary

This summary is machine-generated.

This study developed a rapid, one-pot nucleic acid detection assay combining NASBA amplification with CRISPR-Cas13a for point-of-care pathogen testing. The optimized assay demonstrates high sensitivity for detecting viral RNA, offering a promising tool for infectious disease diagnostics.

Keywords:

CRISPR-Cas NASBA ODE modeling POC pathogen tests

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Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

Area of Science:

Biotechnology
Molecular Diagnostics
CRISPR Technology

Background:

Laboratory-based nucleic acid diagnostics face scaling limitations.
Point-of-care (POC) technologies are crucial for rapid pathogen detection.
Isothermal amplification combined with CRISPR detection offers promising POC solutions.

Purpose of the Study:

To develop and optimize a one-pot NASBA-Cas13a assay for rapid, programmable POC pathogen detection.
To establish design rules for NASBA primers and Cas13a guide RNAs for sensitive viral RNA detection.
To understand the NASBA-Cas13a system through experimental screening and mechanistic modeling.

Main Methods:

Developed an in-house formulation of the Nucleic Acid Sequence-Based Amplification (NASBA) method.
Designed NASBA primer sets and Leptotrichia buccalis Cas13a (LbuCas13a) guide RNAs for SARS-CoV-2 detection.
Utilized high-throughput assay screening and ordinary differential equation (ODE) modeling for system analysis.

Main Results:

Achieved 20-200 aM sensitivity for detecting SARS-CoV-2 viral RNA fragments.
Demonstrated successful optimization of individual NASBA components.
Gained mechanistic understanding of the NASBA-Cas13a reaction scheme.

Conclusions:

The developed NASBA-Cas13a assay provides a sensitive and rapid method for POC nucleic acid detection.
The framework combining experimental screening and ODE modeling aids in understanding and optimizing nucleic acid detection systems.
This approach is valuable for developing future nucleic acid-based diagnostic technologies.