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Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

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Endoplasmic Reticulum-Targeting Delayed Fluorescent Probe for Dual-mode Nitroreductase Sensing.

V T K Shradha¹, Subhadeep Das¹, Abhijit Patra¹

¹Department of Chemistry, Indian Institute of Science Education and Research Bhopal, Bhopal, 462066, Madhya Pradesh, India.

Chemistry, an Asian Journal

|December 13, 2024

Summary

New organic probes enable sensitive, dual-mode detection of cancer biomarkers. These probes utilize delayed fluorescence for specific imaging in cancer cells, overcoming limitations of existing materials.

Keywords:

Endoplasmic reticulum Fluorescence imaging Nitroreductase sensing Thermally activated delayed fluorescence Time-resolved fluorescence imaging

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Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

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Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

Published on: March 14, 2019

Area of Science:

Organic chemistry
Biomedical imaging
Cancer biomarker detection

Background:

Organic thermally activated delayed fluorescence (TADF) materials offer long-lived emission for time-resolved imaging.
Challenges include air sensitivity, lack of organelle specificity, and limited analyte recognition for cancer biomarkers like nitroreductase (NTR).

Purpose of the Study:

To develop novel donor-acceptor based probes for specific and sensitive detection of cancer biomarkers.
To overcome limitations of existing TADF materials for biological applications.

Main Methods:

Synthesis of triphenylamine-quinoxaline based probes (TPQS and TPNS) functionalized with a sulphonamide unit.
Utilizing time-resolved fluorescence measurements to confirm delayed fluorescence properties.
Developing a nitroreductase (NTR) specific probe (TPNS) with a "turn-on" luminescence mechanism.

Main Results:

TPQS probe exhibits delayed fluorescence due to a minimal singlet-triplet energy gap.
TPNS probe shows "turn-on" luminescence and delayed fluorescence upon reaction with NTR.
Dual-mode detection of NTR achieved in cancer cells using confocal and time-resolved fluorescence imaging (TRFI).

Conclusions:

Developed probes demonstrate potential for sensitive and specific detection of cancer biomarkers.
Delayed fluorescent emitters show promise for advanced bioimaging in complex biological systems.
Functionalized probes offer endoplasmic reticulum specificity and precise analyte recognition.